Laser capture microdissection in 2-D co-culture models as a tool to study tumor-stroma interactions.
نویسندگان
چکیده
منابع مشابه
Cell Separation and Gene Expression Analysis in a Tumor-stroma Interaction Model
A novel technique for co-culturing and separating fibroblasts and carcinoma cells in a 2-D model of tumorstroma interaction is presented. The methodology is based on cell co-cultivation on an 1.35 μm thin membrane followed by rapid immunostaining and microdissection of the different cell compartments using a laser microdissection system (P.A.L.M. Microlaser Technologies AG, Germany). For identi...
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عنوان ژورنال:
- BioTechniques
دوره 33 3 شماره
صفحات -
تاریخ انتشار 2002